Agarose–starch gel electrophoresis of rat serum lipoproteins
Rat serum lipoproteins were separated into at least four fractions by agarose–starch gel electrophoresis. The system used was discontinuous in that glycine and sodium barbitone buffer was used in the reservoirs and Tris buffer was used for the gels. The four major bands could be related to the patte...
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1971-05-01
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Series: | Journal of Lipid Research |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520395055 |
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doaj-fc4a5805706e4c5e863202cb647979e02021-04-24T05:52:05ZengElsevierJournal of Lipid Research0022-22751971-05-01123265269Agarose–starch gel electrophoresis of rat serum lipoproteinsA. CHALVARDJIAN0Research Institute, The Hospital for Sick Children, Toronto 101, Ontario, CanadaRat serum lipoproteins were separated into at least four fractions by agarose–starch gel electrophoresis. The system used was discontinuous in that glycine and sodium barbitone buffer was used in the reservoirs and Tris buffer was used for the gels. The four major bands could be related to the pattern obtained by ultracentrifugation. The high density lipoproteins consisted of at least two poorly resolved bands and were not separated from albumin.The vertical gel apparatus was further modified to accept 0.4 ml of rat plasma, which was prestained with Sudan black. After electrophoresis the different lipoprotein bands could conveniently be cut out and the lipid phosphorus determined. The addition of Sudan black B decreased the recovery of the low and high density lipoproteins by 5–9%. However, the recovery of phospholipids was reproducible (80 ± 2%) and the high density lipoproteins contained over two-thirds of the plasma lipid phosphorus.http://www.sciencedirect.com/science/article/pii/S0022227520395055discontinuous system analytical, preparative, ultracentrifugal correlationprestained |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
A. CHALVARDJIAN |
spellingShingle |
A. CHALVARDJIAN Agarose–starch gel electrophoresis of rat serum lipoproteins Journal of Lipid Research discontinuous system analytical, preparative, ultracentrifugal correlation prestained |
author_facet |
A. CHALVARDJIAN |
author_sort |
A. CHALVARDJIAN |
title |
Agarose–starch gel electrophoresis of rat serum lipoproteins |
title_short |
Agarose–starch gel electrophoresis of rat serum lipoproteins |
title_full |
Agarose–starch gel electrophoresis of rat serum lipoproteins |
title_fullStr |
Agarose–starch gel electrophoresis of rat serum lipoproteins |
title_full_unstemmed |
Agarose–starch gel electrophoresis of rat serum lipoproteins |
title_sort |
agarose–starch gel electrophoresis of rat serum lipoproteins |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
1971-05-01 |
description |
Rat serum lipoproteins were separated into at least four fractions by agarose–starch gel electrophoresis. The system used was discontinuous in that glycine and sodium barbitone buffer was used in the reservoirs and Tris buffer was used for the gels. The four major bands could be related to the pattern obtained by ultracentrifugation. The high density lipoproteins consisted of at least two poorly resolved bands and were not separated from albumin.The vertical gel apparatus was further modified to accept 0.4 ml of rat plasma, which was prestained with Sudan black. After electrophoresis the different lipoprotein bands could conveniently be cut out and the lipid phosphorus determined. The addition of Sudan black B decreased the recovery of the low and high density lipoproteins by 5–9%. However, the recovery of phospholipids was reproducible (80 ± 2%) and the high density lipoproteins contained over two-thirds of the plasma lipid phosphorus. |
topic |
discontinuous system analytical, preparative, ultracentrifugal correlation prestained |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520395055 |
work_keys_str_mv |
AT achalvardjian agarosestarchgelelectrophoresisofratserumlipoproteins |
_version_ |
1721511647163449344 |