Including Protons in Solid-State NMR Resonance Assignment and Secondary Structure Analysis: The Example of RNA Polymerase II Subunits Rpo4/7

Including Protons in Solid-State NMR Resonance Assignment and Secondary Structure Analysis: The Example of RNA Polymerase II Subunits Rpo4/7

1H-detected solid-state NMR experiments feasible at fast magic-angle spinning (MAS) frequencies allow accessing 1H chemical shifts of proteins in solids, which enables their interpretation in terms of secondary structure. Here we present 1H and 13C-detected NMR spectra of the RNA polymerase subunit...

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Bibliographic Details
Main Authors: Anahit Torosyan, Thomas Wiegand, Maarten Schledorn, Daniel Klose, Peter Güntert, Anja Böckmann, Beat H. Meier
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-10-01
Series:Frontiers in Molecular Biosciences
Subjects:
Rpo4/7
solid-state NMR
carbon and proton assignments
secondary chemical shifts
ssFLYA
Online Access:https://www.frontiersin.org/article/10.3389/fmolb.2019.00100/full

Internet

https://www.frontiersin.org/article/10.3389/fmolb.2019.00100/full

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