Protocol for in vitro BCR-mediated plasma cell differentiation and purification of chromatin-associated proteins

Protocol for in vitro BCR-mediated plasma cell differentiation and purification of chromatin-associated proteins

Summary: Molecular-level understanding of plasma cell (PC) differentiation has been modeled using lipopolysaccharide (LPS) stimulation in vitro. However, this system does not involve the B-cell receptor (BCR)—a critical component of B cell biology. Here, we present a protocol for in vitro PC differe...

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Bibliographic Details
Main Authors: Kyoko Ochiai, Hiroki Shima, Tsuyoshi Ikura, Marissa C. Franke, Evelyn P. Sievert, Roger Sciammas, Kazuhiko Igarashi
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Antibody
Cell Biology
Cell Differentiation
Chromatin immunoprecipitation (ChIP)
Flow Cytometry/Mass Cytometry
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003403

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http://www.sciencedirect.com/science/article/pii/S2666166721003403

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