Dissolution dynamic nuclear polarization NMR studies of enzyme kinetics: Setting up differential equations for fitting to spectral time courses

Dissolution dynamic nuclear polarization NMR studies of enzyme kinetics: Setting up differential equations for fitting to spectral time courses

ABSTRACT: Dissolution dynamic nuclear polarization (dDNP) provides strikingly increased sensitivity for detecting NMR-receptive nuclei in molecules that are substrates of enzymes and membrane transport proteins. This paves the way for studying the kinetics of many such catalyzed reactions on previou...

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Bibliographic Details
Main Authors: Philip W. Kuchel, Dmitry Shishmarev
Format: Article
Language:English
Published: Elsevier 2019-12-01
Series:Journal of Magnetic Resonance Open
Subjects:
Bloch-McConnell equations
13C NMR spectroscopy
Enzyme kinetics
Dynamic nuclear polarization
Kinetic modeling
Mathematica
Online Access:http://www.sciencedirect.com/science/article/pii/S2666441020300017

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http://www.sciencedirect.com/science/article/pii/S2666441020300017

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