Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells
Background. Metabolic Syndrome (MetS), a major worldwide concern for the public health system, refers to a cluster of key metabolic components, and represents a risk factor for diabetes and cardiovascular diseases. As oxidative stress (OxS) and inflammation are the major triggers of insulin sensitiv...
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doaj-fd17b2b480fa446da9186dc2b108c3412020-11-25T02:07:51ZengMDPI AGNutrients2072-66432020-04-01121175117510.3390/nu12041175Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 CellsMathilde Foisy-Sauvé0Lena Ahmarani1Edgard Delvin2Alain T. Sané3Schohraya Spahis4Emile Levy5Research Centre, CHU Ste-Justine, Montreal, Quebec, H3T 1C5, CanadaResearch Centre, CHU Ste-Justine, Montreal, Quebec, H3T 1C5, CanadaResearch Centre, CHU Ste-Justine, Montreal, Quebec, H3T 1C5, CanadaResearch Centre, CHU Ste-Justine, Montreal, Quebec, H3T 1C5, CanadaResearch Centre, CHU Ste-Justine, Montreal, Quebec, H3T 1C5, CanadaResearch Centre, CHU Ste-Justine, Montreal, Quebec, H3T 1C5, CanadaBackground. Metabolic Syndrome (MetS), a major worldwide concern for the public health system, refers to a cluster of key metabolic components, and represents a risk factor for diabetes and cardiovascular diseases. As oxidative stress (OxS) and inflammation are the major triggers of insulin sensitivity (IS), a cardinal MetS feature, the principal aim of the present work is to determine whether glycomacropeptide (GMP), a milk-derived bioactive peptide, exerts beneficial effects on their expression.<span style="text-transform: uppercase;"> </span>Methods.<span style="text-transform: uppercase;"> </span>Fully differentiated <span style="background: white;">intestinal Caco-2/15 cells are used to evaluate the preventive action of 2 mg/mL GMP against OxS and inflammation induced by the mixture iron-ascorbate (Fe/Asc</span>)<span style="background: white;"> (</span>200 μM:2 mM)<span style="background: white;">. The potency of GMP of decreasing the production of lipoproteins, including chylomicrons (CM), very-low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) is also assessed. </span>Results. <span style="background: white;">The administration of GMP significantly reduces malondialdehyde, a biomarker of lipid peroxidation, and raises superoxide dismutase 2 and glutathione peroxidase via the induction of the nuclear factor erythroid 2–related factor 2, a transcription factor, which orchestrates cellular antioxidant defenses. Similarly, GMP markedly lowers the inflammatory agents tumor necrosis factor-α and cyclooxygenase-2 via abrogation of the nuclear transcription factor-kB. Moreover, GMP-treated cells show a down-regulation of Fe/Asc-induced mitogen activated protein kinase pathway, suggesting greater IS. Finally, GMP decreases the production of CM, VLDL, and LDL. </span>Conclusions. <span style="background: white;">Our results highlight the effectiveness of GMP in attenuating OxS, inflammation and lipoprotein biogenesis, as well as improving IS, the key</span> components of MetS. Further investigation is needed to elucidate the mechanisms mediating the preventive action of GMP.https://www.mdpi.com/2072-6643/12/4/1175Glycomacropeptideoxidative stressinflammationinsulin sensitivitymetabolic syndromemilk-derived bioactive peptide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mathilde Foisy-Sauvé Lena Ahmarani Edgard Delvin Alain T. Sané Schohraya Spahis Emile Levy |
spellingShingle |
Mathilde Foisy-Sauvé Lena Ahmarani Edgard Delvin Alain T. Sané Schohraya Spahis Emile Levy Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells Nutrients Glycomacropeptide oxidative stress inflammation insulin sensitivity metabolic syndrome milk-derived bioactive peptide |
author_facet |
Mathilde Foisy-Sauvé Lena Ahmarani Edgard Delvin Alain T. Sané Schohraya Spahis Emile Levy |
author_sort |
Mathilde Foisy-Sauvé |
title |
Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells |
title_short |
Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells |
title_full |
Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells |
title_fullStr |
Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells |
title_full_unstemmed |
Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells |
title_sort |
glycomacropeptide prevents iron/ascorbate-induced oxidative stress, inflammation and insulin sensitivity with an impact on lipoprotein production in intestinal caco-2/15 cells |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2020-04-01 |
description |
Background. Metabolic Syndrome (MetS), a major worldwide concern for the public health system, refers to a cluster of key metabolic components, and represents a risk factor for diabetes and cardiovascular diseases. As oxidative stress (OxS) and inflammation are the major triggers of insulin sensitivity (IS), a cardinal MetS feature, the principal aim of the present work is to determine whether glycomacropeptide (GMP), a milk-derived bioactive peptide, exerts beneficial effects on their expression.<span style="text-transform: uppercase;"> </span>Methods.<span style="text-transform: uppercase;"> </span>Fully differentiated <span style="background: white;">intestinal Caco-2/15 cells are used to evaluate the preventive action of 2 mg/mL GMP against OxS and inflammation induced by the mixture iron-ascorbate (Fe/Asc</span>)<span style="background: white;"> (</span>200 μM:2 mM)<span style="background: white;">. The potency of GMP of decreasing the production of lipoproteins, including chylomicrons (CM), very-low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) is also assessed. </span>Results. <span style="background: white;">The administration of GMP significantly reduces malondialdehyde, a biomarker of lipid peroxidation, and raises superoxide dismutase 2 and glutathione peroxidase via the induction of the nuclear factor erythroid 2–related factor 2, a transcription factor, which orchestrates cellular antioxidant defenses. Similarly, GMP markedly lowers the inflammatory agents tumor necrosis factor-α and cyclooxygenase-2 via abrogation of the nuclear transcription factor-kB. Moreover, GMP-treated cells show a down-regulation of Fe/Asc-induced mitogen activated protein kinase pathway, suggesting greater IS. Finally, GMP decreases the production of CM, VLDL, and LDL. </span>Conclusions. <span style="background: white;">Our results highlight the effectiveness of GMP in attenuating OxS, inflammation and lipoprotein biogenesis, as well as improving IS, the key</span> components of MetS. Further investigation is needed to elucidate the mechanisms mediating the preventive action of GMP. |
topic |
Glycomacropeptide oxidative stress inflammation insulin sensitivity metabolic syndrome milk-derived bioactive peptide |
url |
https://www.mdpi.com/2072-6643/12/4/1175 |
work_keys_str_mv |
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