Reference gene validation for RT-qPCR, a note on different available software packages.

Reference gene validation for RT-qPCR, a note on different available software packages.

An appropriate normalization strategy is crucial for data analysis from real time reverse transcription polymerase chain reactions (RT-qPCR). It is widely supported to identify and validate stable reference genes, since no single biological gene is stably expressed between cell types or within cells...

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Bibliographic Details
Main Authors: Ward De Spiegelaere, Jutta Dern-Wieloch, Roswitha Weigel, Valérie Schumacher, Hubert Schorle, Daniel Nettersheim, Martin Bergmann, Ralph Brehm, Sabine Kliesch, Linos Vandekerckhove, Cornelia Fink
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4380439?pdf=render

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http://europepmc.org/articles/PMC4380439?pdf=render

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