Reference gene validation for RT-qPCR, a note on different available software packages.
An appropriate normalization strategy is crucial for data analysis from real time reverse transcription polymerase chain reactions (RT-qPCR). It is widely supported to identify and validate stable reference genes, since no single biological gene is stably expressed between cell types or within cells...
Main Authors: | Ward De Spiegelaere, Jutta Dern-Wieloch, Roswitha Weigel, Valérie Schumacher, Hubert Schorle, Daniel Nettersheim, Martin Bergmann, Ralph Brehm, Sabine Kliesch, Linos Vandekerckhove, Cornelia Fink |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4380439?pdf=render |
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