Enhanced trypsin thermostability in Pichia pastoris through truncating the flexible region

Enhanced trypsin thermostability in Pichia pastoris through truncating the flexible region

Abstract Background High thermostability is required for trypsin to have wider industrial applications. Target mutagenesis at flexible regions has been proved to be an efficient protein engineering method to enhance the protein thermostability. Results The flexible regions in porcine trypsin were pr...

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Bibliographic Details
Main Authors: Lin Liu, Haoran Yu, Kun Du, Zhiyan Wang, Yiru Gan, He Huang
Format: Article
Language:English
Published: BMC 2018-10-01
Series:Microbial Cell Factories
Subjects:
Thermostability
Trypsin
Flexible region
Truncation
Ω-loop
Online Access:http://link.springer.com/article/10.1186/s12934-018-1012-x

Internet

http://link.springer.com/article/10.1186/s12934-018-1012-x

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