Enhanced trypsin thermostability in Pichia pastoris through truncating the flexible region
Abstract Background High thermostability is required for trypsin to have wider industrial applications. Target mutagenesis at flexible regions has been proved to be an efficient protein engineering method to enhance the protein thermostability. Results The flexible regions in porcine trypsin were pr...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2018-10-01
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Series: | Microbial Cell Factories |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12934-018-1012-x |