Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display

Abstract Pioneered exactly 20 years ago, yeast surface display (YSD) continues to take a major role in protein engineering among the high-throughput display methodologies that have been developed to date. The classical yeast display technology relies on tethering an engineered protein to the cell wa...

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Bibliographic Details
Main Authors: Doreen Könning, Harald Kolmar
Format: Article
Language:English
Published: BMC 2018-02-01
Series:Microbial Cell Factories
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12934-018-0881-3