Microfluidic encapsulation of Xenopus laevis cell-free extracts using hydrogel photolithography

Summary: Cell-free extract derived from the eggs of the African clawed frog Xenopus laevis is a well-established model system that has been used historically in bulk aliquots. Here, we describe a microfluidic approach for isolating discrete, biologically relevant volumes of cell-free extract, with m...

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Bibliographic Details
Main Authors: Zachary M. Geisterfer, John Oakey, Jesse C. Gatlin
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302082