Meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain development
Retinoic acid (RA) is a diffusible molecule involved in early forebrain patterning. Its later production in the meninges by the retinaldehyde dehydrogenase RALDH2 coincides with the time of cortical neuron generation. A function of RA in this process has not been adressed directly as Raldh2−/− mouse...
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doaj-fdc198670fc04046929aef010dcd43982021-06-02T09:07:23ZengThe Company of BiologistsBiology Open2046-63902017-02-016214816010.1242/bio.021063021063Meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain developmentCarole Haushalter0Brigitte Schuhbaur1Pascal Dollé2Muriel Rhinn3 Development and Stem Cells Department, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch 67404, France Development and Stem Cells Department, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch 67404, France Development and Stem Cells Department, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch 67404, France Development and Stem Cells Department, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch 67404, France Retinoic acid (RA) is a diffusible molecule involved in early forebrain patterning. Its later production in the meninges by the retinaldehyde dehydrogenase RALDH2 coincides with the time of cortical neuron generation. A function of RA in this process has not been adressed directly as Raldh2−/− mouse mutants are embryonic lethal. Here, we used a conditional genetic strategy to inactivate Raldh2 just prior to onset of its expression in the developing meninges. This inactivation does not affect the formation of the cortical progenitor populations, their rate of division, or timing of differentiation. However, migration of late-born cortical neurons is delayed, with neurons stalling in the intermediate zone and exhibiting an abnormal multipolar morphology. This suggests that RA controls the multipolar-to-bipolar transition that occurs in the intermediate zone and allows neurons to start locomotion in the cortical plate. Our work also shows a role for RA in cortical lamination, as deep layers are expanded and a subset of layer IV neurons are not formed in the Raldh2-ablated mutants. These data demonstrate that meninges are a source of extrinsic signals important for cortical development.http://bio.biologists.org/content/6/2/148RetinoidsCerebral cortexNeuronsRadial migrationCortical layering |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carole Haushalter Brigitte Schuhbaur Pascal Dollé Muriel Rhinn |
spellingShingle |
Carole Haushalter Brigitte Schuhbaur Pascal Dollé Muriel Rhinn Meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain development Biology Open Retinoids Cerebral cortex Neurons Radial migration Cortical layering |
author_facet |
Carole Haushalter Brigitte Schuhbaur Pascal Dollé Muriel Rhinn |
author_sort |
Carole Haushalter |
title |
Meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain development |
title_short |
Meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain development |
title_full |
Meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain development |
title_fullStr |
Meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain development |
title_full_unstemmed |
Meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain development |
title_sort |
meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain development |
publisher |
The Company of Biologists |
series |
Biology Open |
issn |
2046-6390 |
publishDate |
2017-02-01 |
description |
Retinoic acid (RA) is a diffusible molecule involved in early forebrain patterning. Its later production in the meninges by the retinaldehyde dehydrogenase RALDH2 coincides with the time of cortical neuron generation. A function of RA in this process has not been adressed directly as Raldh2−/− mouse mutants are embryonic lethal. Here, we used a conditional genetic strategy to inactivate Raldh2 just prior to onset of its expression in the developing meninges. This inactivation does not affect the formation of the cortical progenitor populations, their rate of division, or timing of differentiation. However, migration of late-born cortical neurons is delayed, with neurons stalling in the intermediate zone and exhibiting an abnormal multipolar morphology. This suggests that RA controls the multipolar-to-bipolar transition that occurs in the intermediate zone and allows neurons to start locomotion in the cortical plate. Our work also shows a role for RA in cortical lamination, as deep layers are expanded and a subset of layer IV neurons are not formed in the Raldh2-ablated mutants. These data demonstrate that meninges are a source of extrinsic signals important for cortical development. |
topic |
Retinoids Cerebral cortex Neurons Radial migration Cortical layering |
url |
http://bio.biologists.org/content/6/2/148 |
work_keys_str_mv |
AT carolehaushalter meningealretinoicacidcontributestoneocorticallaminationandradialmigrationduringmousebraindevelopment AT brigitteschuhbaur meningealretinoicacidcontributestoneocorticallaminationandradialmigrationduringmousebraindevelopment AT pascaldolle meningealretinoicacidcontributestoneocorticallaminationandradialmigrationduringmousebraindevelopment AT murielrhinn meningealretinoicacidcontributestoneocorticallaminationandradialmigrationduringmousebraindevelopment |
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