PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus

PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus

The Pragmatic Insertional Mutation Mapping (PIMMS) laboratory protocol was developed alongside various bioinformatics packages (Blanchard et al 2015) to enable detection of essential and conditionally essential genes in Streptococcus and related bacteria. This extended the methodology commonly used...

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Bibliographic Details
Main Authors: Adam Blanchard, Sharon Egan, Richard D Emes, Andrew Warry, James A Leigh
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-10-01
Series:Frontiers in Microbiology
Subjects:
Mutagenesis
Streptococcus
Laboratory protocol
Insertion Sequencing
essential genome
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01645/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01645/full

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