Implication of lipoprotein associated phospholipase A2 activity in oxLDL uptake by macrophages

• Main Authors: Konstantinos P. Markakis, Maria K. Koropouli, Stavroula Grammenou-Savvoglou, Ewoud C. van Winden, Andromaxi A. Dimitriou, Constantinos A. Demopoulos, Alexandros D. Tselepis, Eleni E. Kotsifaki
• Format: Article
• Language: English
• Published: Elsevier 2010-08-01
• Series: Journal of Lipid Research
• Subjects: oxidized 1-palmitoyl-2-arachidonoyl-phosphatidylcholine; lysophosphatidylcholine; moderately oxidized LDL; heavily oxidized LDL
• Online Access: http://www.sciencedirect.com/science/article/pii/S0022227520370553

Recognition and uptake of oxidized LDL (oxLDL) by scavenger receptors of macrophages and foam cell formation are mediated by the oxidatively modified apolipoprotein B (ApoB) and lipid moiety of oxLDL. A great amount of oxidized phosphatidylcholine (oxPC) of oxLDL is hydrolyzed at the sn-2 position by lipoprotein associated phospholipase A2 (Lp-PLA2) to lysophosphatidylcholine and small oxidation products. This study examines the involvement of Lp-PLA2 in the uptake of oxLDL by mouse peritoneal macrophages. LDL with intact Lp-PLA2 activity [LDL (+)] and LDL with completely inhibited Lp-PLA2 activity [LDL (-)] were subjected to oxidation with 5 μM CuSO4 for 6 h [moderately oxLDL (MoxLDL)], or 24 h [heavily oxLDL (HoxLDL)] and peritoneal macrophages were incubated with these preparations. The uptake of MoxLDL(-) was about 30% increased compared with that of MoxLDL(+), and HoxLDL(-) uptake was about 20% increased compared with that of HoxLDL(+). Inhibition of Lp-PLA2 activity had no effect on the uptake of ApoB-liposomes conjugates with ApoB isolated from MoxLDL(-), MoxLDL(+), HoxLDL(-), and HoxLDL(+). Liposomes prepared from the lipid extract of MoxLDL(-), MoxLDL(+), HoxLDL(-), and HoxLDL(+) exhibited a similar pattern to that observed in the uptake of the corresponding intact lipoproteins. This study suggests that the progressive inactivation of Lp-PLA2 during LDL oxidation leads to an increased uptake of oxLDL by macrophages, which could be primarily attributed to the increased uptake of the oxidized phospholipids enriched lipid moiety of oxLDL.