Expanding the Plant GSTome Through Directed Evolution: DNA Shuffling for the Generation of New Synthetic Enzymes With Engineered Catalytic and Binding Properties

Expanding the Plant GSTome Through Directed Evolution: DNA Shuffling for the Generation of New Synthetic Enzymes With Engineered Catalytic and Binding Properties

Glutathione transferases (GSTs, EC. 2.5.1.18) are inducible multifunctional enzymes that are essential in the detoxification and degradation of toxic compounds. GSTs have considerable biotechnological potential. In the present work, a new method for the generation of synthetic GSTs was developed. Ab...

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Bibliographic Details
Main Authors: Evangelia G. Chronopoulou, Anastassios C. Papageorgiou, Farid Ataya, Irini Nianiou-Obeidat, Panagiotis Madesis, Nikolaos E. Labrou
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-11-01
Series:Frontiers in Plant Science
Subjects:
glutathione transferase
directed evolution
DNA shuffling
protein engineering
synthetic biotechnology
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2018.01737/full

Internet

https://www.frontiersin.org/article/10.3389/fpls.2018.01737/full

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