Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data

Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data

Abstract Background The accuracy of microbial community surveys based on marker-gene and metagenomic sequencing (MGS) suffers from the presence of contaminants—DNA sequences not truly present in the sample. Contaminants come from various sources, including reagents. Appropriate laboratory practices...

Full description

Bibliographic Details
Main Authors: Nicole M. Davis, Diana M. Proctor, Susan P. Holmes, David A. Relman, Benjamin J. Callahan
Format: Article
Language:English
Published: BMC 2018-12-01
Series:Microbiome
Subjects:
Microbiome
Metagenomics
Marker-gene
16S rRNA gene
DNA contamination
Online Access:http://link.springer.com/article/10.1186/s40168-018-0605-2

Internet

http://link.springer.com/article/10.1186/s40168-018-0605-2

Similar Items