Parallel Single-Cell RNA-Seq and Genetic Recording Reveals Lineage Decisions in Developing Embryoid Bodies

Parallel Single-Cell RNA-Seq and Genetic Recording Reveals Lineage Decisions in Developing Embryoid Bodies

Summary: Early developmental specification can be modeled by differentiating embryonic stem cells (ESCs) to embryoid bodies (EBs), a heterogeneous mixture of three germ layers. Here, we combine single-cell transcriptomics and genetic recording to characterize EB differentiation. We map transcription...

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Bibliographic Details
Main Authors: Ik Soo Kim, Jingyi Wu, Gilbert J. Rahme, Sofia Battaglia, Atray Dixit, Elizabeth Gaskell, Huidong Chen, Luca Pinello, Bradley E. Bernstein
Format: Article
Language:English
Published: Elsevier 2020-10-01
Series:Cell Reports
Subjects:
embryogenesis
single-cell RNA sequencing
lineage tracing
stem cells
differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124720312110

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http://www.sciencedirect.com/science/article/pii/S2211124720312110

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