Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal

Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal

Abstract Background microRNAs (miRNAs) are short non-coding RNAs that fine-tune gene expression. The aberrant expression of miRNAs is associated with many diseases and they have both therapeutic and biomarker potential. However, our understanding of their usefulness is dependent on the tools we have...

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Bibliographic Details
Main Authors: Rikki A. M. Brown, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Kirsty L. Richardson, Peter J. Leedman
Format: Article
Language:English
Published: BMC 2018-03-01
Series:BMC Biotechnology
Subjects:
microRNAs
microRNA isolation method
Biomarkers
miRNA-based therapy
Extraction
Real-time PCR
Online Access:http://link.springer.com/article/10.1186/s12896-018-0421-6

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http://link.springer.com/article/10.1186/s12896-018-0421-6

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