Amplicon sequencing of colorectal cancer: variant calling in frozen and formalin-fixed samples.

Next generation sequencing (NGS) is an emerging technology becoming relevant for genotyping of clinical samples. Here, we assessed the stability of amplicon sequencing from formalin-fixed paraffin-embedded (FFPE) and paired frozen samples from colorectal cancer metastases with different analysis pip...

Full description

Bibliographic Details
Main Authors: Johannes Betge, Grainne Kerr, Thilo Miersch, Svenja Leible, Gerrit Erdmann, Christian L Galata, Tianzuo Zhan, Timo Gaiser, Stefan Post, Matthias P Ebert, Karoline Horisberger, Michael Boutros
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4444292?pdf=render