Site-Specific Incorporation of Unnatural Amino Acids into Escherichia coli Recombinant Protein: Methodology Development and Recent Achievement

More than two decades ago a general method to genetically encode noncanonical or unnatural amino acids (NAAs) with diverse physical, chemical, or biological properties in bacteria, yeast, animals and mammalian cells was developed. More than 200 NAAs have been incorporated into recombinant proteins b...

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Bibliographic Details
Main Authors: Sviatlana Smolskaya, Yaroslav A. Andreev
Format: Article
Language:English
Published: MDPI AG 2019-06-01
Series:Biomolecules
Subjects:
Online Access:https://www.mdpi.com/2218-273X/9/7/255