Bacterial diversity assessment in Antarctic terrestrial and aquatic microbial mats: a comparison between bidirectional pyrosequencing and cultivation.

The application of high-throughput sequencing of the 16S rRNA gene has increased the size of microbial diversity datasets by several orders of magnitude, providing improved access to the rare biosphere compared with cultivation-based approaches and more established cultivation-independent techniques...

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Bibliographic Details
Main Authors: Bjorn Tytgat, Elie Verleyen, Dagmar Obbels, Karolien Peeters, Aaike De Wever, Sofie D'hondt, Tim De Meyer, Wim Van Criekinge, Wim Vyverman, Anne Willems
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24887330/pdf/?tool=EBI