Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta)

Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta)

In vitro culture of spermatogonial stem cells (SSCs) has generally been performed using two-dimensional (2D) culture systems; however, such cultures have not led to the development of complete spermatogenesis. It seems that 2D systems do not replicate optimal conditions of the seminiferous tubules (...

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Bibliographic Details
Main Authors: Mahmoud Huleihel, Seyedmehdi Nourashrafeddin, Tony M Plant
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2015-01-01
Series:Asian Journal of Andrology
Subjects:
in vitro spermatogenesis; methylcellulose culture system; monkey; primates; soft agar culture system; three-dimensional culture system
Online Access:http://www.ajandrology.com/article.asp?issn=1008-682X;year=2015;volume=17;issue=6;spage=972;epage=980;aulast=Huleihel

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http://www.ajandrology.com/article.asp?issn=1008-682X;year=2015;volume=17;issue=6;spage=972;epage=980;aulast=Huleihel

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