Combined In-Fermenter Extraction and Cross-Flow Microfiltration for Improved Inclusion Body Processing
In this study we demonstrate a new in-fermenter chemical extraction procedure that degrades the cell wall of Escherichia coli and releases inclusion bodies (IBs) into the fermentation medium. We then prove that cross-flow microfiltration can be used to remove 91% of soluble contaminants from the rel...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley-Blackwell,
2004-01.
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| Subjects: | |
| Online Access: | Get fulltext |