Combined In-Fermenter Extraction and Cross-Flow Microfiltration for Improved Inclusion Body Processing
In this study we demonstrate a new in-fermenter chemical extraction procedure that degrades the cell wall of Escherichia coli and releases inclusion bodies (IBs) into the fermentation medium. We then prove that cross-flow microfiltration can be used to remove 91% of soluble contaminants from the rel...
Main Authors: | Chew, Tin Lee (Author), Morreale, Giacomo (Author), Middelberg, Anton P. J. (Author) |
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Format: | Article |
Language: | English |
Published: |
Wiley-Blackwell,
2004-01.
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Subjects: | |
Online Access: | Get fulltext |
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