Construction of a strep-tag II mutant maltose binding protein for reagentless fluorescence sensing
Maltose binding protein (MBP) changes its conformational structure upon its ligand binding.This molecular recognition element that transduces a ligand-binding event into a physical one make MBP an ideal candidate for reagentless fluorescence sensing. MBP gene, (malE) was amplified from a pMaL-C4x pl...
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| Format: | Thesis |
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2012-11.
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| Online Access: | Get fulltext |