| Summary: | Digestive bio-regulator or probiotic is defined as live bacteria with clinically documented health effects in humans and animals. Nowadays, there is increasing interest in probiotics from all over the world. However, the cost of producing probiotics products is still high. To reduce the cost, there is a need to study the usage of agro waste as an inexpensive substrate. The viability of the cell was counted in colony unit per mL (CFU/mL) and the growth was measured using dry weight measurement (g/mL). The sugar concentration was measured using glucose analyzer. At initial substrate concentration of 20% (w/v) and at incubation time of 10hr, the viability cell was 3 x 108 CFU/mL and cell dry weight was 0.0076 g/mL. From the results of this study, it is found that when the initial substrate concentration increased, the viability and growth of Lactobacillus casei increased.
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