Cloning-free CRISPR

Cloning-free CRISPR

We present self-cloning CRISPR/Cas9 (scCRISPR), a technology that allows for CRISPR/Cas9-mediated genomic mutation and site-specific knockin transgene creation within several hours by circumventing the need to clone a site-specific single-guide RNA (sgRNA) or knockin homology construct for each targ...

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Bibliographic Details
Main Authors: Arbab, Mandana (Author), Srinivasan, Sharanya (Contributor), Geijsen, Niels (Author), Sherwood, Richard I (Author), Hashimoto, Tatsunori Benjamin (Contributor)
Other Authors: Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory (Contributor), Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor)
Format: Article
Language:English
Published: Elsevier, 2016-01-13T16:56:19Z.
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