Cloning-free CRISPR
We present self-cloning CRISPR/Cas9 (scCRISPR), a technology that allows for CRISPR/Cas9-mediated genomic mutation and site-specific knockin transgene creation within several hours by circumventing the need to clone a site-specific single-guide RNA (sgRNA) or knockin homology construct for each targ...
Main Authors: | , , , , |
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Other Authors: | , |
Format: | Article |
Language: | English |
Published: |
Elsevier,
2016-01-13T16:56:19Z.
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Subjects: | |
Online Access: | Get fulltext |