Rationally engineered Cas9 nucleases with improved specificity
The RNA-guided endonuclease Cas9 is a versatile genome-editing tool with a broad range of applications from therapeutics to functional annotation of genes. Cas9 creates double-strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide. However, Cas9 can cleave off-target sites...
Main Authors: | , , , , , |
---|---|
Other Authors: | , , , |
Format: | Article |
Language: | English |
Published: |
American Association for the Advancement of Science (AAAS),
2016-05-22T23:25:15Z.
|
Subjects: | |
Online Access: | Get fulltext |