Rationally engineered Cas9 nucleases with improved specificity

Rationally engineered Cas9 nucleases with improved specificity

The RNA-guided endonuclease Cas9 is a versatile genome-editing tool with a broad range of applications from therapeutics to functional annotation of genes. Cas9 creates double-strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide. However, Cas9 can cleave off-target sites...

Full description

Bibliographic Details
Main Authors: Slaymaker, Ian (Contributor), Gao, Linyi (Contributor), Zetsche, Bernd (Contributor), Scott, David Arthur (Contributor), Yan, Winston Xia (Contributor), Zhang, Feng (Contributor)
Other Authors: Harvard University- (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: American Association for the Advancement of Science (AAAS), 2016-05-22T23:25:15Z.
Subjects:
Article
Online Access:Get fulltext

Internet

Get fulltext

Similar Items