Rationally engineered Cas9 nucleases with improved specificity
The RNA-guided endonuclease Cas9 is a versatile genome-editing tool with a broad range of applications from therapeutics to functional annotation of genes. Cas9 creates double-strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide. However, Cas9 can cleave off-target sites...
Main Authors: | Slaymaker, Ian (Contributor), Gao, Linyi (Contributor), Zetsche, Bernd (Contributor), Scott, David Arthur (Contributor), Yan, Winston Xia (Contributor), Zhang, Feng (Contributor) |
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Other Authors: | Harvard University- (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor) |
Format: | Article |
Language: | English |
Published: |
American Association for the Advancement of Science (AAAS),
2016-05-22T23:25:15Z.
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Subjects: | |
Online Access: | Get fulltext |
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