BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis

BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis

Enhancers, critical determinants of cellular identity, are commonly recognized by correlative chromatin marks and gain-of-function potential, although only loss-of-function studies can demonstrate their requirement in the native genomic context. Previously, we identified an erythroid enhancer of hum...

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Main Authors: Canver, Matthew C. (Author), Smith, Elenoe C. (Author), Sher, Falak (Author), Pinello, Luca (Author), Shalem, Ophir (Contributor), Chen, Diane D. (Author), Schupp, Patrick G. (Author), Vinjamur, Divya S. (Author), Garcia, Sara P. (Author), Luc, Sidinh (Author), Kurita, Ryo (Author), Nakamura, Yukio (Author), Fujiwara, Yuko (Author), Maeda, Takahiro (Author), Yuan, Guo-Cheng (Author), Zhang, Feng (Contributor), Orkin, Stuart H. (Author), Bauer, Daniel E. (Author), Sanjana, Neville (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2016-05-22T23:55:16Z.
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