High-throughput functional genomics using CRISPR-Cas9

High-throughput functional genomics using CRISPR-Cas9

Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenot...

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Bibliographic Details
Main Authors: Shalem, Ophir (Contributor), Zhang, Feng (Contributor), Sanjana, Neville (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2016-05-23T00:03:50Z.
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