Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

Targeted genome editing technologies have enabled a broad range of research and medical applications. The Cas9 nuclease from the microbial CRISPR-Cas system is targeted to specific genomic loci by a 20 nt guide sequence, which can tolerate certain mismatches to the DNA target and thereby promote und...

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Bibliographic Details
Main Authors: Ran, F. Ann (Author), Hsu, Patrick D (Author), Lin, Chie Yu (Contributor), Gootenberg, Jonathan S. (Author), Konermann, Silvana M. (Contributor), Trevino, Alexandro E. (Author), Scott, David A. (Author), Inoue, Azusa (Author), Matoba, Shogo (Author), Zhang, Yi (Author), Zhang, Feng (Contributor), Ran, F. Ann (Contributor), Scott, David Arthur (Contributor), Hsu, Patrick D. (Contributor)
Other Authors: Institute for Medical Engineering and Science (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: Cell Press/Elsevier, 2016-06-01T17:36:15Z.
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