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|a dc
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|a Rohani, Nazanin
|e author
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|a Massachusetts Institute of Technology. Department of Biological Engineering
|e contributor
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|a Massachusetts Institute of Technology. Department of Biology
|e contributor
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|a Koch Institute for Integrative Cancer Research at MIT
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|a Oudin, Madeleine Julie
|e contributor
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|a Hughes, Shannon K.
|e contributor
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|a Rohani, Nazanin
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|a Moufarrej, Mira N.
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|a Lauffenburger, Douglas A.
|e contributor
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|a Gertler, Frank
|e contributor
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|a Moufarrej, Mira N.
|e author
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|a Jones, Joan G.
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|a Condeelis, John S.
|e author
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|a Gertler, Frank B.
|e author
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|a Oudin, Madeleine Julie
|e author
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|a Gertler, Frank
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|a Hughes-Alford, Shannon Kay
|e author
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|a Lauffenburger, Douglas A
|e author
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|a Characterization of the expression of the pro-metastatic MenaINV isoform during breast tumor progression
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|b Springer Netherlands,
|c 2016-07-14T21:18:56Z.
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| 856 |
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|z Get fulltext
|u http://hdl.handle.net/1721.1/103617
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|a Several functionally distinct isoforms of the actin regulatory Mena are produced by alternative splicing during tumor progression. Forced expression of the Mena[superscript INV] isoform drives invasion, intravasation and metastasis. However, the abundance and distribution of endogenously expressed Mena[superscript INV] within primary tumors during progression remain unknown, as most studies to date have only assessed relative mRNA levels from dissociated tumor samples. We have developed a Mena[superscript INV] isoform-specific monoclonal antibody and used it to examine Mena[superscript INV]expression patterns in mouse mammary and human breast tumors. Mena[superscript INV] expression increases during tumor progression and to examine the relationship between Mena[superscript INV] expression and markers for epithelial or mesenchymal status, stemness, stromal cell types and hypoxic regions. Further, while Mena[superscript INV] robustly expressed in vascularized areas of the tumor, it is not confined to cells adjacent to blood vessels. Altogether, these data demonstrate the specificity and utility of the anti-Mena[superscript INV]-isoform specific antibody, and provide the first description of endogenous Mena[superscript INV]protein expression in mouse and human tumors.
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|a United States. Dept. of Defense. Breast Cancer Research Program (Grants W81XWH-10-1-0040 and W81XWH-13-1-0031)
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|a National Institutes of Health (U.S.) (Grants U54-CA112967 and GM58801)
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|a Massachusetts Institute of Technology. Ludwig Center for Molecular Oncology
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|a en
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|a Article
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|t Clinical & Experimental Metastasis
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