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|a Xie, Shicong
|e author
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|a Massachusetts Institute of Technology. Computational and Systems Biology Program
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|a Massachusetts Institute of Technology. Department of Biology
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|a Xie, Shicong
|e contributor
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|a Mason, Frank M
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|a Martin, Adam C
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|a Mason, Frank M
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|a Martin, Adam C
|e author
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|a Loss of G[subscript α12/13] exacerbates apical area-dependence of actomyosin contractility
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|a Loss of Gα12/13 exacerbates apical area dependence of actomyosin contractility
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|b American Society for Cell Biology,
|c 2016-11-04T20:05:57Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/105213
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|a During development, coordinated cell shape changes alter tissue shape. In the Drosophila ventral furrow and other epithelia, apical constriction of hundreds of epithelial cells folds the tissue. Genes in the G[subscript α12/13] pathway coordinate collective apical constriction, but the mechanism of coordination is poorly understood. Coupling live-cell imaging with a computational approach to identify contractile events, we discovered that differences in constriction behavior are biased by initial cell shape. Disrupting G[subscript α12/13] exacerbates this relationship. Larger apical area is associated with delayed initiation of contractile pulses, lower apical E-cadherin and F-actin levels, and aberrantly mobile Rho-Kinase structures. Our results suggest that loss of G[subscript α12/13] disrupts apical actin cortex organization and pulse initiation in a size-dependent manner. We propose that G[subscript α12/13] robustly organizes the apical cortex despite variation in apical area to ensure the timely initiation of contractile pulses in a tissue with heterogeneity in starting cell shape.
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|a en_US
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|a Article
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|t Molecular Biology of the Cell
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