Viral Packaging and Cell Culture for CRISPR-Based Screens
This protocol describes how to perform the tissue culture and high-throughput sequencing library preparation for a CRISPR-based screen. First, pantropic lentivirus is prepared from a sgRNA plasmid pool and applied to the target cells. Following antibiotic selection and a harvest of the initial popul...
| Main Authors: | , , |
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| Other Authors: | , , |
| Format: | Article |
| Language: | English |
| Published: |
Cold Spring Harbor Laboratory Press,
2016-11-18T18:38:57Z.
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| Subjects: | |
| Online Access: | Get fulltext |