Large-Scale Single Guide RNA Library Construction and Use for CRISPR-Cas9-Based Genetic Screens
The ability to systematically disrupt genes serves as a powerful tool for understanding their function. The programmable CRISPR-Cas9 system enables efficient targeting of large numbers of genes through the use of single guide RNA (sgRNA) libraries. In cultured mammalian cells, collections of knockou...
| Main Authors: | , , |
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| Other Authors: | , , |
| Format: | Article |
| Language: | English |
| Published: |
Cold Spring Harbor Laboratory Press,
2016-12-22T15:06:50Z.
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| Subjects: | |
| Online Access: | Get fulltext |