Large-Scale Single Guide RNA Library Construction and Use for CRISPR-Cas9-Based Genetic Screens

• Main Authors: Wang, Tim (Contributor), Lander, Eric Steven (Contributor), Sabatini, David (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor), Whitehead Institute for Biomedical Research (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor)
• Format: Article
• Language: English
• Published: Cold Spring Harbor Laboratory Press, 2016-12-22T15:06:50Z.
• Subjects: Article
• Online Access: Get fulltext

The ability to systematically disrupt genes serves as a powerful tool for understanding their function. The programmable CRISPR-Cas9 system enables efficient targeting of large numbers of genes through the use of single guide RNA (sgRNA) libraries. In cultured mammalian cells, collections of knockout mutants can be readily generated by means of transduction of Cas9-sgRNA lentiviral pools, screened for a phenotype of interest, and counted using high-throughput DNA sequencing. This technique represents the first general method for undertaking systematic loss-of-function genetic screens in mammalian cells. Here, we introduce the methodology and rationale for conducting CRISPR-based screens, focusing on distinguishing positive and negative selection strategies.
National Institutes of Health (U.S.) (Grant CA103866)
National Human Genome Research Institute (U.S.) (Grant 2U54HG003067-10)
Broad Institute of MIT and Harvard
National Science Foundation (U.S.)