Site-specific protein modification using immobilized sortase in batch and continuous-flow systems
Transpeptidation catalyzed by sortase A allows the preparation of proteins that are site-specifically and homogeneously modified with a wide variety of functional groups, such as fluorophores, PEG moieties, lipids, glycans, bio-orthogonal reactive groups and affinity handles. This protocol describe...
| Main Authors: | , , , , , , , , , |
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| Other Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Springer Nature,
2017-03-07T19:21:07Z.
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| Subjects: | |
| Online Access: | Get fulltext |