Fluorophore-Conjugated Holliday Junctions for Generating Super-Bright Antibodies and Antibody Fragments

Fluorophore-Conjugated Holliday Junctions for Generating Super-Bright Antibodies and Antibody Fragments

The site-specific modification of proteins with fluorophores can render a protein fluorescent without compromising its function. To avoid self-quenching from multiple fluorophores installed in close proximity, we used Holliday junctions to label proteins site-specifically. Holliday junctions enable...

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Bibliographic Details
Main Authors: Theile, Christopher S. (Author), Chen, Guan-Yu (Author), Bilate, Angelina M. (Author), Duarte, Joao N. (Author), Avalos, Ana M. (Author), Fang, Tao (Author), Barberena, Roberto (Author), Sato, Shuji (Author), Ploegh, Hidde (Contributor), Li, Zeyang,S.M.Massachusetts Institute of Technology (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor), Whitehead Institute for Biomedical Research (Contributor)
Format: Article
Language:English
Published: Wiley Blackwell, 2017-05-01T18:47:33Z.
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