Site-specific protein modification using immobilized sortase in batch and continuous-flow systems
Transpeptidation catalyzed by sortase A allows the preparation of proteins that are site-specifically and homogeneously modified with a wide variety of functional groups, such as fluorophores, PEG moieties, lipids, glycans, bio-orthogonal reactive groups and affinity handles. This protocol describe...
Main Authors: | , , , , , , , , , |
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Other Authors: | |
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group,
2017-05-26T14:48:49Z.
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Subjects: | |
Online Access: | Get fulltext |