A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks

A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks

Finding the components of cellular circuits and determining their functions systematically remains a major challenge in mammalian cells. Here, we introduced genome-wide pooled CRISPR-Cas9 libraries into dendritic cells (DCs) to identify genes that control the induction of tumor necrosis factor (Tnf)...

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Main Authors: Parnas, Oren (Author), Jovanovic, Marko (Author), Eisenhaure, Thomas M (Author), Herbst, Rebecca H (Author), Ye, Chun Jimmie (Author), Przybylski, Dariusz (Author), Tirosh, Itay (Author), Satija, Rahul (Author), Raychowdhury, Raktima (Author), Mertins, Philipp (Author), Carr, Steven A (Author), Hacohen, Nir (Author), Regev, Aviv (Author), Dixit, Atray C. (Contributor), Platt, Randall Jeffrey (Contributor), Sanjana, Neville E (Contributor), Shalem, Ophir (Contributor), Zhang, Feng (Contributor)
Other Authors: Institute for Medical Engineering and Science (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: Elsevier, 2017-06-30T17:51:03Z.
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