Optimized protocol for expression and purification of membrane-bound PglB, a bacterial oligosaccharyl transferase

Asparagine-linked glycosylation (NLG) plays a significant role in a diverse range of cellular processes, including protein signaling and trafficking, the immunologic response, and immune system evasion by pathogens. A major impediment to NLG-related research is an incomplete understanding of the cen...

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Bibliographic Details
Main Authors: Jaffee, Marcie Beth (Contributor), Imperiali, Barbara (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Chemistry (Contributor)
Format: Article
Language:English
Published: Elsevier, 2017-06-30T18:34:04Z.
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