High-throughput nuclear delivery and rapid expression of DNA via mechanical and electrical cell-membrane disruption

High-throughput nuclear delivery and rapid expression of DNA via mechanical and electrical cell-membrane disruption

Nuclear transfection of DNA into mammalian cells is challenging yet critical for many biological and medical studies. Here, by combining cell squeezing and electric-field-driven transport in a device that integrates microfluidic channels with constrictions and microelectrodes, we demonstrate nuclear...

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Bibliographic Details
Main Authors: Ding, Xiaoyun Sean (Contributor), Stewart, Martin P (Contributor), Sharei, Armon Reza (Contributor), Weaver, James C (Contributor), Langer, Robert S (Contributor), Jensen, Klavs F (Contributor)
Other Authors: Harvard University- (Contributor), Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor), Jensen, Klavs F. (Contributor)
Format: Article
Language:English
Published: 2017-08-28T17:18:50Z.
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