Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ

Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ

Background: Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration into the target chromosome (PITCh) system for a gene...

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Bibliographic Details
Main Authors: Aida, Tomomi (Contributor), Nakade, Shota (Author), Sakuma, Tetsushi (Author), Izu, Yayoi (Author), Oishi, Ayu (Author), Mochida, Keiji (Author), Ishikubo, Harumi (Author), Usami, Takako (Author), Aizawa, Hidenori (Author), Yamamoto, Takashi (Author), Tanaka, Kohichi (Author)
Other Authors: McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: Biomed Central Ltd, 2017-12-13T16:34:18Z.
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