Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ
Background: Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration into the target chromosome (PITCh) system for a gene...
Main Authors: | Aida, Tomomi (Contributor), Nakade, Shota (Author), Sakuma, Tetsushi (Author), Izu, Yayoi (Author), Oishi, Ayu (Author), Mochida, Keiji (Author), Ishikubo, Harumi (Author), Usami, Takako (Author), Aizawa, Hidenori (Author), Yamamoto, Takashi (Author), Tanaka, Kohichi (Author) |
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Other Authors: | McGovern Institute for Brain Research at MIT (Contributor) |
Format: | Article |
Language: | English |
Published: |
Biomed Central Ltd,
2017-12-13T16:34:18Z.
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Subjects: | |
Online Access: | Get fulltext |
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