Engineered Cpf1 variants with altered PAM specificities

Engineered Cpf1 variants with altered PAM specificities

The RNA-guided endonuclease Cpf1 is a promising tool for genome editing in eukaryotic cells. However, the utility of the commonly used Acidaminococcus sp. BV3L6 Cpf1 (AsCpf1) and Lachnospiraceae bacterium ND2006 Cpf1 (LbCpf1) is limited by their requirement of a TTTV protospacer adjacent motif (PAM)...

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Main Authors: Schneider, Martin W (Author), Yamano, Takashi (Author), Nishimasu, Hiroshi (Author), Nureki, Osamu (Author), Crosetto, Nicola (Author), Gao, Linyi (Contributor), Cox, David Benjamin Turitz (Contributor), Yan, Winston Xia (Contributor), Manteiga, John Colonnese (Contributor), Zhang, Feng (Contributor)
Other Authors: Institute for Medical Engineering and Science (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2018-01-29T16:03:54Z.
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