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|a Reczek, Colleen R
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|a Koch Institute for Integrative Cancer Research at MIT
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|a Wang, Tim
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|a Sabatini, David
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|a Martínez-Reyes, Inmaculada
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|a Sabatini, David
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|a A CRISPR screen identifies a pathway required for paraquat-induced cell death
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|b Nature Publishing Group,
|c 2018-07-03T19:03:49Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/116772
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|a Paraquat, a herbicide linked to Parkinson's disease, generates reactive oxygen species (ROS), which causes cell death. Because the source of paraquat-induced ROS production remains unknown, we conducted a CRISPR-based positive-selection screen to identify metabolic genes essential for paraquat-induced cell death. Our screen uncovered three genes, POR (cytochrome P450 oxidoreductase), ATP7A (copper transporter), and SLC45A4 (sucrose transporter), required for paraquat-induced cell death. Furthermore, our results revealed POR as the source of paraquat-induced ROS production. Thus, our study highlights the use of functional genomic screens for uncovering redox biology.
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|t Nature Chemical Biology
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