A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes

A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes

Apicomplexan parasites are leading causes of human and livestock diseases such as malaria and toxoplasmosis, yet most of their genes remain uncharacterized. Here, we present the first genome-wide genetic screen of an apicomplexan. We adapted CRISPR/Cas9 to assess the contribution of each gene from t...

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Bibliographic Details
Main Authors: Sidik, Saima M. (Author), Huet, Diego (Author), Huynh, My-Hang (Author), Thiru, Prathapan (Author), Saeij, Jeroen P.J (Author), Carruthers, Vern B. (Author), Lourido, Sebastian (Author), Ganesan, Suresh M. (Contributor), Nasamu, Armiyaw Sebastian (Contributor), Niles, Jacquin (Contributor), Wang, Tim (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Whitehead Institute for Biomedical Research (Contributor)
Format: Article
Language:English
Published: Elsevier BV, 2018-09-11T15:22:47Z.
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