Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9

Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9

The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets bearing a protospacer adjacent motif (PAM) and complementarity to an RNA guide. Unlike other Cas9 orthologs, Corynebacterium diphtheriae Cas9 (CdCas9) recognizes the promiscuous NNRHHHY PAM. However, the CdCas9-mediated PAM rec...

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Bibliographic Details
Main Authors: Hirano, Seiichi (Author), Abudayyeh, Omar O. (Author), Gootenberg, Jonathan S (Author), Horii, Takuro (Author), Ishitani, Ryuichiro (Author), Hatada, Izuho (Author), Zhang, Feng (Author), Nishimasu, Hiroshi (Author), Nureki, Osamu (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: Springer Science and Business Media LLC, 2019-11-08T21:59:25Z.
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