Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9
The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets bearing a protospacer adjacent motif (PAM) and complementarity to an RNA guide. Unlike other Cas9 orthologs, Corynebacterium diphtheriae Cas9 (CdCas9) recognizes the promiscuous NNRHHHY PAM. However, the CdCas9-mediated PAM rec...
Main Authors: | Hirano, Seiichi (Author), Abudayyeh, Omar O. (Author), Gootenberg, Jonathan S (Author), Horii, Takuro (Author), Ishitani, Ryuichiro (Author), Hatada, Izuho (Author), Zhang, Feng (Author), Nishimasu, Hiroshi (Author), Nureki, Osamu (Author) |
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Other Authors: | Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor) |
Format: | Article |
Language: | English |
Published: |
Springer Science and Business Media LLC,
2019-11-08T21:59:25Z.
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Subjects: | |
Online Access: | Get fulltext |
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